A longitudinal study on PRRSV detection in swine herds with different demographics and PRRSV management strategies.

Porcine reproductive and respiratory syndrome virus (PRRSV) has been one of the major health-related concerns in the swine production industry. Through its rapid transmission and mutation, the simultaneous circulation of multiple PRRSV strains can be a challenge in PRRSV diagnostic, control and surveillance. The objective of this longitudinal study was to describe the temporal detection of PRRSV in swine farms with different production types and PRRS management strategies. Tonsil scraping (n = 344) samples were collected from three breeding and two growing herds for approximately one year. In addition, processing fluids (n = 216) were obtained from piglet processing batches within the three breeding farms while pen-based oral fluids (n = 125) were collected in the two growing pig farms. Viral RNA extraction and reverse-transcription quantitative PCR (RT-qPCR) were conducted for all samples. The sample positivity threshold was set at quantification cycle (Cq) of ≤ 37. Statistical analyses were performed using generalized linear modelling and post hoc pairwise comparisons with Bonferroni adjustments using R statistical software. The results suggested a higher probability of detection in processing fluids compared to tonsil scraping specimens [odds ratio (OR) = 3.86; p = .096] in breeding farms whereas oral fluids were outperformed by tonsil scrapings (OR = 0.26; p < .01) in growing pig farms. The results described herein may lead to an improvement in PRRSV diagnostic and surveillance by selecting proper specimens.

Evaluation of a Water-Based Medium-Expansion Foam Depopulation Method in Suckling and Finisher Pigs.

The threat of foreign animal disease introduction through contaminated animal products, feed ingredients, and wildlife vectors have highlighted the need for additional approved methods for mass depopulation of swine under emergency scenarios, especially methods that can be applied to pigs across all production phases. The market disruption within the swine industry due to the SARS-CoV-2 pandemic has demonstrated this lack of preparation. The objective of this study was to validate water-based foam as a mass depopulation method for suckling (18 to 24 days of age) and finisher stage (63 to 100 days of age) pigs. Finisher pigs (n = 31, originally 32 but one finisher pig died prior to foaming), allocated as 9 triads and 1 set of 4 pigs, in 10 total replicates, and suckling pigs (n = 32), randomly allocated to two replicates, were completely covered in water-based medium-expansion foam for a 15-min dwell time in a bulk container. Container fill time for the trials were 6.5 ± 0.68 s and 5.3 ± 0.03 s for finisher and suckling pig replicates, respectively. Average (± SD) time for cessation of movement was 105 ± 39.1 s (s) for finisher pigs and 79.5 ± 10.5 s for suckling pigs. After completion of the 15-min dwell time in the foam, all pigs were confirmed dead upon removal from the container. The results from the present study suggest that the use of water-based foam can be an effective means of mass depopulation for suckling and finisher stage pigs, supporting previous research on the application to adult swine.

Risk factors and impact of COVID-19-related clinic closures on the detection of gastrointestinal parasites in dogs, a cross-sectional study.

Infections with endoparasites, especially gastrointestinal helminths, are a common finding in client-owned dogs. The Community Practice section at the Ohio State University Veterinary Medical Center (OSU-VMC) follows Companion Animal Parasite Council, American Animal Hospital Association, and American Veterinary Medical Association guidelines for parasitology by recommending annual fecal analyses for dogs and prescribing year-round, broad-spectrum parasite preventatives. There is increasing interest in determining if parasite occurrence is changing in client-owned dogs. Therefore, a retrospective study was designed to examine risk factors associated with the detection of parasites in samples submitted to the OSU-VMC Clinical Veterinary Parasitology Diagnostic Laboratory. Of the 1198 canine fecal samples, 254 (21.2%) of these samples had a positive fecal analysis for gastrointestinal (GI) parasites. The age of the dog, time of year, reproductive status, purpose of fecal examination, GI signs, and type of parasite preventatives were assessed as potential risk factors for GI parasite infection in dogs. To determine if Coronavirus Disease 2019 (COVID-19)-associated hospital restrictions impacted the number of fecal examinations performed, data from January to December 2020, was compared to the same period in 2019. There was nearly a 50% reduction in canine fecal samples submitted to the OSU-VMC Clinical Veterinary Parasitology Diagnostic Laboratory in March 2020 compared to March 2019. At least one canine GI parasite was found in over 20% of all 1198 canine fecal samples (21.2%, 254/1198). The most commonly detected canine GI parasite in all the fecal samples was hookworm at 9.6% (115/1198), followed by Giardia sp. at 7.6% (91/1198). Age, use of parasite preventatives, breed, and reproductive status were found to be associated with parasite occurrence in the dog samples. Identifying such risk factors in dogs will guide veterinarians to advise annual fecal examinations more strongly to clients with high-risk dogs or when routine health visits are postponed for an extended period.

One health research ethics

Emerging and re-emerging infectious diseases (EIDs) present major threats to public health, global security, and economic development. Coronavirus disease 2019 (COVID-19) is the latest EID to demonstrate the devastation, suffering, and scale of death that an EID can cause. Pandemics involving emerging and re-emerging infectious agents and associated infectious diseases, climate change, urbanization, biodiversity loss and financial instability have been identified as the most critical global issues today (1). Close to three-quarters of today's EIDs are known to be of zoonotic origin (where infectious agents spread to humans from domestic or wild animals), and their frequency and economic impact are on the rise

A Systematic Literature Review on Depopulation Methods for Swine.

Swine mass depopulation refers to the destruction of large numbers of pigs and may include not only animals affected with a disease but also healthy pigs in a facility or surrounding areas. Emerging applications of mass depopulation include reducing welfare issues associated with slaughter delays, which was observed in the United States in 2020 as a result of the Coronavirus disease (COVID-19) pandemic. The objectives of this review were to summarize the available literature on swine depopulation methods and to highlight critical gaps in knowledge. Peer-reviewed articles were identified through a systematic search in electronic databases including Web of Science, MEDLINE, and PubMed. A total of 68 publications were assessed. Gaseous carbon dioxide inhalation was the most commonly reported depopulation method for both small- and large-scale trials. Measurements of consciousness state, which serves to assess suffering and humaneness, appeared to be lacking in a high proportion of the studies. None of the published studies demonstrated an ideally reliable and safe way to induce rapid unconsciousness in large groups of pigs. Development of rapid mass depopulation methods applicable to large groups of pigs is necessary to provide industry partners with suitable and low-cost emergency preparedness procedures while adhering to personnel safety and animal welfare standards. Lastly, there is an urgent need to standardize comprehensive reporting guidelines for depopulation studies.